Quantitation of the inflammatory cell infiltrate in the lungs of rMA15 virus infected STAT1−/− mice at 8dpi.

Analysis of inflammatory cell infiltrates during rMA15 infection of STAT1−/− mice. Lymphocytes from lungs of 129 WT and STAT1−/− mice infected with rMA15 or PBS for 8 days, were isolated and labeled with antibodies to identify inflammatory cell populations by flow cytometry. Macrophages were identified as CD11c−/CD11b+/GR-1int/MHCII+ cells, neutrophils as F480−/CD11b+/CD11c−/GR-1+ cells and eosinophils as CD11c−/Siglec+/GR-1− cells. The percent of total starting lymphocytes is shown in the table for each cell type.