Activation of the 2′-N-Acetyltransferase Gene [aac(2′)-Ia] in Providencia stuartii by an Interaction of AarP with the Promoter Region

The aac(2′)-Ia gene in Providencia stuartii encodes a 2′-N-acetyltransferase capable of acetylating both peptidoglycan and certain aminoglycoside antibiotics. Regulation of the aac(2′)-Ia gene is influenced in a positive manner by the product of the aarP gene, which encodes a small transcriptional activator of the AraC (XylS) family. In this study, we demonstrate the sequence requirements at the aac(2′)-Ia promoter for AarP binding and activation.