Regulation of Parietal Cell Homeostasis by Bone Morphogenetic Protein signaling

Loss of BMP signaling in the stomach, achieved by transgenic expression of the BMP inhibitor noggin (H+/K+-Nog mice), causes parietal cells (PC) loss, SPEM, a marker of pre-neoplasia, and activation of cell proliferation. Purpose: We examined if specific inhibition of BMP signaling in PCs leads to aberrations in epithelial homeostasis Overall design: Isolation of PCs from control and Nog-expressing mice, was achieved by crossing H+/K+-Nog mice to Rosa26-tdTomato (Tom) mice to generate H+/K+-Nog;Rosa26-tdTom mice. H+/K+-Cre mice were then crossed to H+/K+-Nog;Rosa26-tdTom mice to generate H+/K+-Cre;H+/K+-Nog;Rosa26-tdTom mice. Tom-labeled PCs were purified by flow-cytometry.