Wheat spikelet LC-MSMS - iTRAQ-based quantitative proteome analysis reveals metabolic changes between a cleistogamous wheat mutant and its wild-type wheat counterpart

A novel wheat mutant, ZK001, characterized as cleistogamic and presumed to provide structural barriers for Fusarium head blight during the flowering stage, was created through static magnetic fields mutagenesis from YM18. The atrophied, thin and ineffective lodicules of ZK001 lost the ability to push the lemma and palea apart during the flowering stage. Compared to YM18, ZK001 exhibited lower Fusarium infection. comparative studies at the proteome level were employed between YM18 and ZK001. isobaric tags for relative and absolute quantitation (iTRAQ) and liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) were employed to characterize protein expression differences between YM18 and ZK001.