TFIIS-mediated recovery of HIV-1 elongation from arrest

TFIIS reactivates arrested RNA Pol II directly interacting with the enzyme resulting in endonucleolytic excision of nascent transcript ~7-14 nucleotides upstream of the 3' end. This reaction is catalyzed by the catalytic site and results in the generation of a new 3'-OH terminus that could be used for re-extension from the correctly base paired site (reviewed by Shilatifard et al., 2003).

TFIIS直接重新激活被抑制的RNA聚合酶II，导致新生转录本的端核苷酸切割，切割点位于3'端上游约7-14个核苷酸处。此反应由催化位点催化，从而生成一个新的3'-OH末端，该末端可用于从正确配对的碱基位点重新延伸（参见Shilatifard等人，2003年的综述）。