PRO-seq assay in adult ventricular cardiomyocytes

PRO-seq assay was performed for the quantitative detection of nascent pre-mRNA transcripts induced by phenylephrine (PE) and adenoviral overexpression of SRF serine-103 mutants in adult ventricular cardiomyocytes本研究采用PRO-seq测定法（PRO-seq assay），对成年心室心肌细胞中由苯肾上腺素（PE）以及腺病毒介导的SRF丝氨酸103突变体过表达所诱导的新生前体mRNA转录本开展定量检测。