Conidia were inoculated into 200 ml medium at a concentration of 106 conidia/ml, and incubated at 37°C with shaking (200 rpm) for 36 h. The cell wall was extracted as described under Materials and Met
Evidence is now accumulating that sub-populations of ribosomes - so-called specialized ribosomes - can favour the translation of subsets of mRNAs. Here we use a large collection of diploid yeast strai
We homogenize the cellular 40S ribosomes through deleting the whole set of alternative duplicated genes encoding yeast 40S RP paralogs. This strain(Homo-40S) shows atrong sensitivity to the translatio
xls-file pEV: negative control strainPsppA-mCherry: plasmid based expression of mCherry from the inducible promoter PsppAPslpA-mCherry: plasmid based expression of mCherry from the constitutive
