Modeling Glioma Oncostreams In Vitro: Spatiotemporal Dynamics of their Formation, Stability, and Disassembly

Movie 1 of 16 is a supplementary material for the manuscript 'Modeling Glioma Oncostreams In Vitro: Spatiotemporal Dynamics of their Formation, Stability, and Disassembly.' It visually demonstrates the effect of various treatments on the formation and dynamics of oncostreams in high-grade glioma cells. The time-lapse videos provide insightful observations into how various pharmacological drugs influence the initial formation and structural dynamics of oncostreams." It aims to enhance the understanding of the foundational stages of oncostream formation in glioma cells and their response to various pharmacological treatments, supporting the findings discussed in the manuscript. Individual Movie Descriptions: Movie 1: Low Density Oncostream Dynamics: Demonstrates the formation and dynamics of oncostreams in GFP+ NPA glioma cells at low seeding density (1 × 10^5 cells), over 24 hours using time-lapse confocal imaging. Movie 2: High Density Oncostream Dynamics: Explores the effect of high cell seeding density (2 × 10^5 cells) on the formation and behavior of oncostreams in GFP+ NPA glioma cells, analyzed over 24 hours. Movie 3: Spatiotemporal Progression of Oncostreams: Showcases the sequential self-formation and developmental stages of oncostreams over a 24-hour period. Movie 4: Collagenase Impact on Oncostreams: Description: Illustrates the disassembly of aggressive and malignant oncostreams with 15 U/ml collagenase treatment over 15 hours. Movie 5: TC-I-15 Inhibition of Oncostream Formation: Details the impact of TC-I-15, an integrin antagonist, on adhesion and oncostream formation in glioma cells, recorded over 20 hours. Movie 6: Cytochalasin D Disruption of Oncostreams: Captures the effect of Cytochalasin D on oncostream formation by inhibiting actin polymerization, observed over a 1-hour period with rapid imaging intervals. Movie 7: Myosin II Inhibition in Oncostreams with p-nitro Blebbistatin: Presents the influence of p-nitro Blebbistatin on oncostream formation by inhibiting myosin II, documented over 1 hour with dynamic cellular responses. Movie 8: Control - Oncostream Formation without BAPTA-AM: Demonstrates oncostream formation and dynamics without BAPTA-AM treatment, monitored over 16 hours. Movie 9: BAPTA-AM (Calcium Modulation) Impact on Oncostream Formation: Reveals the effects of BAPTA-AM treatment on oncostream dynamics, observed over a 16-hour period. Movie 10: Glutamate Influence on Oncostreams: Shows the effects of glutamate treatment on oncostream formation and dynamics, monitored over 16 hours. Movie 11: Histamine Impact on Oncostream Dynamics: Demonstrates the influence of histamine on the formation and behavior of oncostreams, captured over a 16-hour period. Movie 12: Oncostream Formation on Non-Laminin Coated Surfaces: Illustrates the compromised organization of oncostreams on poly-D-lysine coated dishes without laminin, observed over 45 hours. Movie 13: 4-HAP Treatment Effect on Oncostreams: Shows the impact of 4-HAP treatment on the structure of oncostreams, monitored over 20 hours. Movie 14: Rho-Activator I Untreated Control: Presents the formation and dynamics of oncostreams without Rho-Activator I treatment, recorded over 16 hours. Movie 15: Rho-Activator I Influence on Oncostreams Dynamics: Details the effects of Rho-Activator I on oncostream formation and dynamics, observed over a 16-hour period. Movie 16: Rho-Inhibitor Effect on Oncostreams Dynamics: Demonstrates the impact of Rho-Inhibitor on the formation and behavior of oncostreams, monitored over 16 hours.