RNA-seq of gonadal adipose tissue from male and female Trim28-WT and Trim28 adipose specific KO mice

Many genes have been implicated in WAT lipid metabolism, including tripartite motif containing 28 (Trim28), a gene proposed to primarily influence adiposity via epigenetic mechanisms in embryonic development. We set out to determine if adipose specific deletion of Trim28 led to changes in adipose tissue function and molecular phenotype. We performed transcriptomics analysis on adipose tissue taken from WT and adipose specific Trim28 KO mice to investigate their molecular phenotype, and to identify pathways altered in KO animals.已有诸多基因被证实与白色脂肪组织（White Adipose Tissue, WAT）的脂质代谢密切相关，其中包括三元基序包含28（tripartite motif containing 28, Trim28），该基因被认为主要通过胚胎发育过程中的表观遗传机制影响脂肪堆积。本研究旨在探究Trim28的脂肪组织特异性敲除是否会改变脂肪组织的功能与分子表型。我们对采集自野生型（Wild Type, WT）及脂肪组织特异性Trim28敲除（Knock Out, KO）小鼠的脂肪组织开展转录组学分析，以探究二者的分子表型，并鉴定敲除小鼠体内发生改变的信号通路。