poly(A+) RNAs 3'-seq analysis in control and U1-specific proteins KD (knock-down) samples

To understand the effect of U1 snRNP-specific proteins on poly(A+) RNAs profiles, we carried out control and U1A, U1C, U1-70k depletion (using synthetic siRNAs) experiments followed by poly(A+) RNA 3'-seq anlyais using lexogen kit (016.024)in HeLa cells Overall design: siRNAs targeting U1A, U1-70K, U1C were designed and transfected into Hela cells,knock-down efficiency was confirmed by western blots. 3'-seq analysis was performed using lexogen kit (016.024)