Fluorophores and fluorescence microscopy

When utilizing a scanning confocal microscope, better resolution and image contrast is achieved when compared to traditional methods. Thin optical sections of living specimens, 100 micrometers thick can be imaged with such devices, which are capable of examining fluorescence emission between 400nm and 750nm. By using laser light of wavelength 470nm as an excitation source, a root cell sample stained with two different fluorescent dyes was examined. Two distinct peaks were notated at 643.98nm (red) and 537.72nm (green). The fluorophores used were deemed to be monolignol (green) and Peridinin Chlorophyll (red) as they matched the ratio of excitation/emission wavelength de-tected in the experiment.

与传统显微成像技术相比，使用扫描共聚焦显微镜（scanning confocal microscope）可获得更优异的分辨率与图像对比度。该类设备可对厚度为100微米的活体标本薄光学切片进行成像，且能够检测400nm至750nm波段的荧光发射信号。本研究以波长470nm的激光作为激发光源（excitation source），对经两种不同荧光染料染色的根细胞样本开展检测。实验中记录到两个清晰的荧光发射峰，分别位于643.98nm（红光）与537.72nm（绿光）处。由于实验检测到的激发/发射波长比值与二者匹配，因此所用荧光团（fluorophores）被确定为单木质醇（monolignol，对应绿光信号）与多甲藻素-叶绿素（Peridinin Chlorophyll，对应红光信号）。

搜集汇总

背景与挑战

背景概述

该数据集包含使用扫描共聚焦显微镜对根细胞样本进行荧光检测的实验数据，检测了两种荧光染料（绿色为monolignol，红色为Peridinin Chlorophyll）的发射波长。数据集包括一个Excel文件和一个PDF报告，适用于荧光显微镜技术研究。