ChIP-seq analysis of ELK3 in T-ALL cells

ELK3 is responsible for encoding a member within the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily.To identify downstream target genes directly regulated by ELK3 and reveal its critical role in T-ALL, we performed immunoprecipitation analysis using ELK3 antibodies in KOPTK1 cells Fragmentation of the genome of KOPTK1 was achieved using ultrasonication, followed by immunoprecipitation with ELK3 antibodies (Novus)