Metabolic disorder and gut microbiota feature in high energy diet

To study dynamic and diversied impacts of fat and sugar. In our current study, we investigated the dynamic changes of gut microbiota, and host metabolism in mice that were fed with either chow diet, HFD or chow diet with 30% sucrose in drinking water (HSD) for continued 12 weeks. The gut microbiota was analyzed with 16S rDNA sequencing on feces. Hepatic gene expression profile was tested with transcriptomics analysis on liver tissue. The host metabolism was evaluated by measuring body weight, insulin sensitivity, serum lipids and expression of proteins involved in lipid metabolism of liver.