Inhibition of recombinant SmPOP by protease inhibitors.

a Abbreviations: PMSF (phenylmethylsulfonyl fluoride), TLCK (Nα-Tosyl-L-lysine chloromethyl ketone), TPCK (N-p-Tosyl-L-phenylalanine chloromethyl ketone), BPTI (bovine pancreatic trypsin inhibitor), STI (soybean trypsin inhibitor), E64 (trans-Epoxysuccinyl-L-leucylamido(4-guanidino)butane)b The target proteases are classified based on catalytic type into aspartic (AP), cysteine (CP) and serine (SP) proteases, and metalloproteases (MP).c The recombinant SmPOP was pre-incubated with the given inhibitor and remaining activity was measured in a kinetic assay with the fluorogenic substrate Z-Gly-Pro-AMC. The mean values ± S.D. of three replicates are expressed as percentage inhibition relative to the uninhibited control.d CMK: chloromethyl ketone; CHO: aldehyde; OH: free carboxyl.Inhibition of recombinant SmPOP by protease inhibitors.