ZSeekerDB: A Database of Z-DNA Sequences Across Organismal Genomes

ZSeekerDB is a comprehensive bioinformatics resource cataloging predicted Z-DNA and Z-RNA sequences across the complete genomes of over 266,000 organisms, spanning all major domains of life.The database was generated using the ZSeeker algorithm, a validated sequence-based predictor of Z-DNA-forming potential, applied to assemblies retrieved from NCBI GenBank and RefSeq. Each record includes chromosomal coordinates, Z-DNA score, and taxonomic metadata, enabling large-scale comparative genomics, evolutionary, and functional analyses. This dataset release (v0.1, 2025-11-05) provides the unified DuckDB database file (zdnadatabase.duckdb, ~58 GB) used by the ZSeekerDB web platform.The file contains core tables for Z-DNA loci, species-level metadata, and pre-materialized SQL views to support efficient analytical queries. Researchers can explore and reproduce the same results programmatically via REST or SQL endpoints documented at the project website.The database architecture is implemented in Go (backend) and React + ECharts (frontend), with DuckDB providing the analytical engine for high-performance, in-process querying.