16S and 23S ribosomal RNA gene fragments sequenced by a MinION nanopore system were used to obtain sub-species resolution of datasets from a D6322 mock bacterial microbiome. Correct strain resolution
C. kwangsiensis RNA was used to construct cDNA libraries with fragment a length of 200 bp. Then, paired-end sequencing was performed using an Illumina HiSeq TM 2500 following the manu
Relatively small volumes of water may contain sufficient environmental DNA (eDNA) to detect target aquatic organisms via genetic sequencing. We therefore assessed the utility of eDNA to document the di
