We report Transcription Activator-like Effector Nucleases (TALEN)-mediated mutagenesis of the Rett Syndrome gene, methyl-CpG binding protein 2 (Mecp2), in non-human primates (NHPs). We conclude that T
sgRNAs were designed using the Merry Crispr algorithm described at: https://github.com/milescsmith/merrycrispr When possible, the goal was to design 6 sgRNAs for each of the 21,574 Macaca mulatta pr
