DRS expression and polyA site measurement of Pcf11 mutations in yeast

Direct-RNA Sequencing expression data, quantifying both abundance and 3'-processing (cleavage and polyadenylation) site was obtained for two distinct mutations of Pcf11 and matched wildtype w303 samples under both standard growth conditions, as well as under growth in a caffeine-supplemented medium, since these mutations have been shown to increase susceptiblity to caffeine-related phenotypes. The overall goal was quantification of expression and pre-mRNA processing changes in response to these mutations. Direct RNA Sequencing data was obtained for 8 yeast samples, including two distinct mutations of Pcf11 (YDR228C), Pcf11-13 and Pcf11-deltaNL, under growth in either YAPD media, or YAPD with inclusion of 20mM caffeine. Resulting sequenced data was processed to get both gene expression and polyA site selection