Assembly of insect SRA accessions - part 1

To identify insect species potentially associated with Ophiocordyceps-related yeast-like symbionts, transcriptomic SRA reads were aligned against the StYLS coding sequences of 18 ribosomal proteins and cytochrome c oxidase subunit I (COI). Read mapping was performed with Magic‑BLAST v1.7.0. Libraries with ≥20 aligned reads were downloaded. Reads were then subjected to quality control using the BBTools suite and assembled with SPAdes v4.2.0 in “meta” mode using default parameters. The resulting transcripts were queried against the NCBI core-nr database using DIAMOND BLASTx to identify fungal transcripts belonging to genera within the family Ophiocordycipitaceae. The first 100 FASTA files from the insect assembled libraries are provided here (please consult 10.5281/zenodo.18507008 for the last 43 files). Filenames contain the insect species name and the NCBI accession number of the analyzed SRA library.