Single-cell chromatin accessibility and lipid profiling reveals SCD1-dependent metabolic shift in adipocytes induced by bariatric surgery

We applied single-cell ATAC sequencing and lipid profiling to inguinal and epididymal adipose depots from mice that received sham surgery or vertical sleeve gastrectomy (VSG). We observed depot-specific cellular composition and chromatin accessibility patterns that were altered by VSG. Specifically, accessibility at Scd1, a fatty acid desaturase, was substantially reduced after VSG in mature adipocytes of inguinal but not epididymal depots. This was accompanied by reduced accumulation of SCD1-produced unsaturated fatty acids. Given these findings and reports that reductions in Scd1 attenuate obesity and insulin resistance our results suggest VSG exerts its beneficial effects through an inguinal depot-specific reduction of SCD1 activity. single-cell chromatin accessibility (ATAC-seq) of inguinal and epididymal adipose depots from mice 2.5 months after a sham surgery or verical sleeve gastrectomy. Please note that the raw fastq.gz files were generated by Illumina pipeline software v2.18, then decomplexed to integrate the barcodes from index1 and index2 reads into the read names of the R1 and R2 files using snATAC decomplex by the Ren lab (https://github.com/r3fang/snATAC). the barcode metadata that lists the barcodes associated with each sample in a file called barcode_metadata.txt.