Interferon-gamma and TNF-alpha synergistically enhance the immunomodulatory capacity of extracellular vesicles from Endometrial-Derived Mesenchymal Stromal Cells

Extracellular vesicles from endometrial-derived mesenchymal stromal cells (EVendMSCs) (n=3) and from endometrial-derived MSCs primed with Interferon-gamma and TNF-alpha (Interferon-gamma/TNF-alpha primed EVendMSCs) (n=3) were characterized by transcriptomic approach. miRNAs were isolated and sequenced by next generation sequencing to evaluate the differential expression between EVendMSCs and Interferon-gamma/TNF-alpha primed EVendMSCs. Interferon-gamma/TNF-alpha primed EVendMSCs may be an innovative therapeutic option in various inflammatory mediated-diseases.