Ago2-RIP-chip in Dicer WT and KO macrophages. Mus musculus

To determine the spectrum of miRNA targets regulated following Dicer deletion, we performed argonaute 2 (AGO2)-RNA Immunoprecipitation (RIP)-microarray in bone marrow-derived macrophages (BMDMs) from LysM-Cre/Dicerflox/flox/Apoe–/– and LysM-Cre/Dicerwt/wt/Apoe–/– mice. This analysis combined with miRNA profiling in Dicer wild type (WT) and knockout (KO) BMDMs may help to identify the miRNA targets regulated by Dicer deletion. Overall design: Bone marrow cells were harvested from femora of 6-8 week old male LysM-Cre/Dicerflox/flox/Apoe–/– or LysM-Cre/Dicerwt/wt/Apoe–/– mice, re-suspended in DMEM-F12/10% FCS/10% L929-conditioned medium, and cultured for 7 days to differentiate into primary macrophages. RNA was isolated before and after AGO2-RIP or IgG control-RIP using Trizol.