Figure S1 Plot Layout

Plot layout of a field experiment conducted from June through August 2011 (Corvallis, OR, USA) to test the
effects of Bacillus thuringiensis (Bt) and non-Bt maize on the colonization ability and community diversity of arbuscular
mycorrhizal fungi (AMF) in roots. Each plot measured 1 m by 1.2 m in size and there was a 1 m unplanted border around all plots. Each plot contained 20 plants (14 different cultivars B1-B9 and P1-P5) and each Bt cultivar was sown next to its non-Bt parental (P) isoline. Corresponding Bt/P pairs are indicated in the plot map as follows: B1/P1 = pink; B2/P2 = yellow; B3/P3 = purple; B4/P4 = gray; B5/P3 = brown; B6/P2 = green; B7/P5 = red; B8/P5 = blue; and B9/P5 = orange. Plant IDs followed by a "T" (in white) were used to trap spores for later experiments and are thus not included in the present study. Plant growth responses and percent AMF colonization in roots were recorded for all plants in the experiment (360 plants). Root samples collected from a subset of plots (2, 8, 10, 14, and 16; outlined in black) were used for molecular analysis of AMF communities using 454 pyrosequencing (90 plants).