Complete Genome Sequencing of MRSA Ocular Isolates from the Patients with Eye Diseases

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most common causes of Bacterial keratitis infection, causing significant morbidity and mortality in developing countries like India. Thus, MRSA ocular infections are diseases of emerging importance, which need our attention for best and effective therapeutics. Identification of its genetic determinants of pathogenesis in ocular infections is hindered by the unavailability of its complete genome sequence. Several studies reported that ONT sequencing is a valuable tool for high-throughput bacterial genome finishing. Here, we report the results of a de novo assembly using Oxford nanopore long reads, complemented with Illumina short reads for polishing and comparative study of clinically significant antibiotic-resistant MRSA Keratitis isolates.