Isoenergetic microarrays probes that bind strongly and moderately to M121 and their thermodynamic properties.

a –binding sites of probes are denoted by the middle nucleotide of the complementary target RNA region, square brackets indicate alternative complementary site of M121 with the same sequence;b- nucleotides in capital letter (A, C, G, U, D) are 2’-O-methyl-RNA nucleotides, nucleotides with L superscript (AL, CL, GL, UL, DL) are locked nucleic acid (LNA) nucleotides, D and DL are 2,6 –diaminopurine riboside (2’-O-methyl type or LNA, respectively);c– Binding was considered strong (S), medium (M) and weak (W), when the integrated intensities were ≥1/3, ≥ 1/9 and ≥ 1/27 of the strongest intensity, no binding was marked as (-). Hybridization condition: buffer: 300 mM KCl, 5 mM MgCl2, 50 mM HEPES, pH 7.0;d- ΔG°37 calculated as modified probe/RNA duplex [36];e- calculated in RNAstructure program as RNA/RNA duplex and, in parenthesis, the site of binding for which calculation was done.Only alternative binding sites of probes with thermodynamic stability more favorable than -4.0 kcal/mol are noted. Binding sites of probes are denoted by the middle nucleotide of the complementary RNA region (or two nucleotides for region with an even number of nucleotides).Isoenergetic microarrays probes that bind strongly and moderately to M121 and their thermodynamic properties.