Interaction between the TCF7L2 gene and dietary intake on metabolic syndrome risk factors among Saudi Arabian adults

Transcription factor-7-like 2 (TCF7L2) is the most critical type 2
diabetes (T2D) gene identified to date. The single-nucleotide polymorphism
(SNP) rs7903146 in TCF7L2 in T2D interacts with dietary factors; however,
research on nutrigenetics among Saudi Arabians is limited. This study
investigated the interaction between the SNP rs7903146 and dietary intake
on factors that may contribute to MetS among Saudi Arabian adults. This
cross-sectional study included 271 adult participants (aged 20–55 years)
of both genders. Anthropometric measurements and dietary assessments using
a food frequency questionnaire were performed. Fasting blood samples were
collected to analyze serum lipid, glucose, and insulin levels. Genetic
analysis was performed using real-time polymerase chain reaction.
Univariate regression was used to examine the association between the
TCF7L2 SNP rs7903146 and laboratory parameters, and to test SNP-diet
interactions. The additive model was used in the analysis and the T allele
was the effect allele. A marginal significant association was observed
between SNP rs7903146 and waist circumference (WC) (P=0.05). Carriers of
the TT genotype had the highest WC (83.5± 20.1 cm) when compared with the
CC genotype (80± 14.2 cm) and the TC genotype (77.9± 13.9 cm). The SNP
rs7903146 was significantly associated with total energy intake (P = 0.04)
and saturated fatty acids (SFA, P = 0.005), and TT carriers had the
highest total energy and SFA consumption (3606.9± 1554.7 kcal, 66.8± 52.0
g, respectively). Only one near significant interaction was observed
between SNP rs7903146 and total energy intake on insulin levels (P=0.04),
with carriers of the TT genotype showing a greater reduction in insulin
values (-5.3± 3.5) at lower energy intake when compared with the CC (-2.4±
3.1), and TC (-4.7± 2.8). No significant interaction was found. The
present study observed significant associations between SNP rs7903146 and
total energy and SFA consumptions. The TT carriers had increased
consumption of total energy and SFA. Future studies using larger sample
sizes are required to confirm a significant interaction between SNP
rs7903146 and diet on factors that may contribute to MetS in the Saudi
population.