Data and code for: Failure to purge: Population and individual inbreeding effects on fitness across generations of wild Impatiens capensis
收藏Mendeley Data2024-04-13 更新2024-06-28 收录
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https://datadryad.org/stash/dataset/doi:10.5061/dryad.jwstqjqf3
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We measured the reproductive output (a component of fitness) of Impatiens capensis plants in each of 12 home field sites (N = 808 individuals). These field sites occurred in fragmented floodplain forests and marches surrounded by an urban/agricultural matrix in Wisconsin, USA. We define reproductive output as the number of cleistogamous and chasmogamous structures on each plant during peak flower (binned into buds, open flowers, and seed capsules and bare pedicles). We also measured the height of each plant. We collected cleistogamous and chasmogamous seeds (N = 6,859) from as many of these mother plants as possible (N = 718 maternal plants with seeds collected). We germinated the seeds in a greenhouse, transplanted the seedlings into a common garden, and measured lifetime fitness (total number of ripe seed capsules produced) of each progeny (N = 2,260) in the common garden. A subset of the maternal plants (N = 296) were genotyped for a previous study (Toczydlowski and Waller, 2019, Molecular Ecology), at thousands of SNP loci using genotype-by-sequencing (1 x 100 base pair reads). We used this SNP dataset to calculate individual maternal multilocus heterozygosity (sMLH), individual inbreeding (f), and population-level inbreeding (FIS ). We then compared the fitness of CH and CL progeny at each major life stage, estimated cumulative inbreeding depression and inbreeding load across the lifetime of the progeny, and tested how maternal sMLH related to both maternal and progeny fitness. Our main goal was to test if inbreeding depression declines in more inbred populations.
创建时间:
2023-06-28



