Thiol-Disulphide Homoeostasis in Covıd-19: Evaluation of its Relationship with Complete Blood Count Parameters

This study was conducted with 68 individuals who applied to Sakarya University Medical Faculty Training and Research Hospital between May 15 and August 15 with positive COVID-19 PCR results, which were treated and followed up in the service and intensive care unit (ICU), as well as any chronic diseases and drugs admitted to the general internal medicine outpatient clinic on the same dates as a control group with negative PCR test results. Individuals with positive COVID-19 PCR results were divided into two groups as inpatients (33) and ICU (35) patients. Within the scope of the study, the patients' age, gender, chronic diseases and clinical information (application complaints, hospitalization in the medical floor-ICU, intubation status, death or discharge status and the drugs they used) were obtained through the hospital automation system. Patients who refused to participate in the study and were under 18 years of age were excluded from the study. Remaining parts of the samples taken during routine analysis were kept under appropriate conditions and no additonal sample was taken.
All values obtained were evaluated in the SPSS (ver. 20.0; SPSS, USA) program. The mean, median, min-max value and standard deviations of the measurement results were calculated. The Shapiro-Wilk Test was used to determine whether the data conformed to normal distribution. Student-t test was used when parametric test conditions were met in groups with two independent variables, Mann-Whitney U test if not provided, One-Way Analysis of Variance if parametric test conditions were met in groups with more than two independent variables, and Kruskal-Wallis test if not. Pearson Chi-Square test was used for categorical variables. Correlation and analysis were performed to evaluate the relationship between thiol-disulfide levels of patients with CBC parameters and clinical course. Significance was assessed at least at the p <0.05 level. In addition, the relationship between TT, NT, DD, lymphocyte, neutrophil, WBC, PLR, NLR parameters and the need for intensive care treatment (prognosis) of the patients was also examined by ROC analysis.

本研究针对68名在2023年5月15日至8月15日期间向萨卡里亚大学医学院培训与研究医院申请并确认COVID-19 PCR检测结果为阳性的个体进行，这些个体在服务部和重症监护室（ICU）接受治疗和随访，同时作为对照组，纳入了在相同日期被收入普通内科门诊的、PCR检测结果为阴性的慢性病患者及药物使用情况。确认COVID-19 PCR检测结果为阳性的个体被分为两组：住院组（33人）和ICU组（35人）。研究范围内，通过医院自动化系统收集了患者的年龄、性别、慢性疾病及临床信息（申请投诉、医疗楼层-ICU住院、插管状态、死亡或出院状态以及所用药物）。拒绝参与研究或年龄低于18岁的个体被排除在研究之外。常规分析过程中采集的样本剩余部分在适宜条件下保存，未额外采集样本。
所有获得的数据均在SPSS（版本20.0；SPSS，美国）程序中进行评估。计算了测量结果的平均值、中位数、最小-最大值和标准偏差。使用Shapiro-Wilk检验确定数据是否符合正态分布。在满足参数检验条件且具有两个独立变量的组中，采用Student-t检验；若不满足，则使用Mann-Whitney U检验。在具有多于两个独立变量的组中，若满足参数检验条件，则采用单因素方差分析；若不满足，则采用Kruskal-Wallis检验。对于分类变量，使用Pearson卡方检验。通过相关性分析和分析，评估了患者血细胞计数参数与临床病程中硫醇-二硫键水平之间的关系。显著性评估至少在p < 0.05水平。此外，通过ROC分析，还考察了TT、NT、DD、淋巴细胞、中性粒细胞、WBC、PLR、NLR参数与患者需要重症监护治疗（预后）之间的关系。