Structural and functional adaptation of Haloferax volcanii TFEa/ß

Purpose: The Haloferax volcanii ?tfeB strain provided a unique opportunity to study a putative role of TFEß in the regulation of gene expression. Results: The deletion of the tfeB gene in H. volcanii results in the aberrant expression of approximately one third of all genes, consistent with its function as a basal transcription initiation factor. Interestingly, tfeB deletion particularly affects foreign genes including a prophage region. Conclusions: Our results confirm the dual function of TFE as basal factor and regulator of transcription Overall design: We isolated total RNA from Haloferax volcanii ?tfeB H2644 and H2645 and the parental H53 strain during early exponential growth (O.D.650=0.2) and subjected it to next generation RNA sequencing (RNA-seq)