Next Generation Sequencing Analysis of human pluripotent stem cells derived endothelial and smooth muscle cells

Purpose: global gene expression profiling of H1 and iPSC derived endothelial cells (ECs) and smooth mucle cells (SMCs). Methods: SMART-seq2 amplified polyA RNA from undifferentiated H1 cells and iPSCs (in biological duplicates), cardiovascular progenitor cells, endothelial cells (ECs) and smooth muscle cells (SMCs) Results: Insulin free condition promoted cardiovascular mesoderm, endothelial and smooth muscle differentiation. Conclusions: the gene expression profiles of ECs and SMCs differentiated in insulin free medium confirmed that they are true ECs and SMCs. Perform RNA-seq of undifferentaited H1 and human iPSCs, day 3 mesoderm progenitor cells, day 8 endothelial and smooth muscle cells.