Identity and characteristics of confirmed EdU incorporation hits: function criteria, cell-cycle expression pattern and activity in related functional screens.

*Function criteria used for inclusion in the lentiviral ORF library, based on published microarray expression pattern or functional screening result: C- cell cycle phase-specific expression; ML-Myeloid cell proliferation [26]; P- phosphoregulation [27], [28]; MR- mitotic regulation [24]; U- unspecified i.e. randomly picked clones.†Cell cycle phase-specific expression determined by microarray analysis in: a, primary human foreskin fibroblasts [22] or HeLa cells b, [25] and c, [23].§Positive (+), negative (−) or neutral (*) effect on cell proliferation detected in either cDNA overexpression screens in: 1, SMMC7721-human hepatoma cells [11]; 2, NIH 3T3 mouse fibroblasts [11]; 3, U2OS-human osteosarcoma cells [9]; or in RNAi-mediated knock-down screens for effects on mitosis detected in 4a- HeLa [29], 4b- Hela [32], 5- HT29-human colon carcinoma [24], and 6- U2OS cells [31].