Small RNA-seq reveals miRNAs that may regulate pupal diapause in the flesh fly, Sarcophaga bullata

Small RNA-seq was used to identify miRNAs in pupal stage Sarcophaga bullata and to assess differences in the abundance of miRNAs in developing and diapause (i.e. dormant) pupae. Overall design: miRNA profiles in diapausing and nondiapausing pupae of the flesh fly, Sarcophaga bullata were discovered with RNA-seq using Illumina GAIIx. Six samples were sequenced with 3 relicate samples for nondiapause (NDH_LD) and 3 diapause (NDH_SD) replicates.