Rat cell supernatant and the separation of supernatant LC-MSMS

We find one organ of body is special useful for skeletal muscle recovery from indury. To identify the active substance in TCM(special organ cell culturing supernatant which can activate satellite cell proliferation), we prepared serum-free TCM and also separate several fractions, subjected them to LC-MS/MS analysis. To our surprise, most proteins extracted belong to metabolite interconversion enzymes, nucleic acid binding proteins, cytoskeletal proteins, protein modifying enzymes, membrane traffic proteins and so on, but no cytokine was detected.