Adrenergic signaling stimulates body-wide stem cell activation for limb regeneration (4N RNA-Seq)

In axolotls, stem cells are systemically prompted to re-enter the cell cycle upon amputation. We sought to identify which cells drive systemic-activation response in the limbs distant to the amputation. Overall design: We dissociated homeostatic or systemically-activated limbs at 14 days post-amputation, and sorted them by their DNA content using FACS. We then performed single-cell RNA sequencing of the dividing, 4C population using 10X genomics.