PMACS-95 (in paper)

Project title: PMACS-95Grant Information: AHA Grant 9TPA34910007, Regulatory role of pericardial macrophages in myocardial infarctionRelevant Citations: https://doi.org/10.1016/j.isci.2024.110244Project Description:· Animal Model: N/A – In Vitro Experiment using Mouse Cells· Animal Age: 8-13 Weeks· Animal Sex: Male· Animal Strain: C57B6/J· Number: 5 Mice· Groups:o Fibroblasts Cultured Alone§ With and Without 100ng/mL LPS Stimulationo Bone marrow derived macrophages alone§ With and Without 100ng/mL LPS Stimulationo Bone Marrow Derived Macrophages co-cultured with Fibroblasts§ With and Without 100ng/mL LPS Stimulationo Bone Marrow Derived Macrophages Cultured on Transwell plate with Fibroblasts on Bottom Plate§ With and Without 100ng/mL LPS Stimulationo Peritoneal Macrophages alone§ With and Without 100ng/mL LPS Stimulationo Peritoneal Macrophages co-cultured with Fibroblasts§ With and Without 100ng/mL LPS Stimulationo Peritoneal Macrophages cultured on Transwell Plate with Fibroblasts on Bottom Plate§ With and Without 100ng/mL LPS Stimulation· Experimental Conditions: Peritoneal fibroblasts, bone marrow derived macrophages, and peritoneal macrophages were all harvested from B6 mice. Fibroblasts were plated at 20,000 cells/well in AMCF media. After 24 hours, macrophages were plated at 30,000 cells/well either alone, on top of fibroblasts, or with transwell in BMDM media. 24-hours later, media removed and replaced with AMCF containing 100 ng/mL LPS. Cells were harvested after 16 hours and examined via qPCR.Licenses: N/A