Kosakonia cowanii strain:Esp_Z Genome sequencing. Kosakonia cowanii strain:Esp_Z

To gain insight into the genetic basis of the Esp_Z-mediated inhibition of Plasmodium and develop tools for bacterial functional genomics, we sequenced the Esp_Z genome via a whole-genome shotgun sequencing approach, as described in Materials and Methods. A total of 864,207 Titanium sequencing reads were obtained, corresponding to an approximately 38X genome coverage. After assembly and ORF prediction, a total of 39 scaffolds were obtained, two of which were identified as plasmid-related through identity with known plasmid sequences in Genbank. PCR and Sanger-based sequencing analysis confirmed the presence of two closed plasmids. The estimated size of the chromosomal Esp_Z genome is then 5.2 Mbp, with a GC-content averaging 55.8%. A phylogenetic analysis based on 16S rRNA genomic sequences allowed for the confirmation of Esp_Z as a novel Enterobacter species. The Esp_Z genome contains 4,919 putative protein-coding genes that were subjected to SEED functional analysis and used to construct microarrays for whole-genome expression analysis.in Dennison & Saraiva et al. (2016). Functional genomic analyses of Enterobacter, Anopheles and Plasmodium reciprocal interactions that impact vector competence. Malaria Journal