qRT-PCR for selected viral RNAs in lymphnodes.
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adetection limit (number of template copies) using DNA template. bdetermined following qPCR using SYBR-green technology; not applicable (n.a.) to conventional PCR, which was detected by EtBR staining after agarose gel electrophoresis of the PCR product. c25 ng RNA template (from lymphnodes of animals with MCF) before reverse transcription. Numbers in the column refer to copy number detected. d25 ng RNA template (from lymphnodes of healthy animals) before reverse transcription.
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2013-02-21
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