Quantifying lesion size, fibroblast and macrophage density at 4 and 28 days post thoracic contusion in young and middle-aged female C57BL/6J Mice

STUDY PURPOSE: We sought to compare young (2-3 months) and middle age (10-12 months) mice in contusive T8 injury outcomes at acute and chronic timepoints. DATA COLLECTED: Injury parameters; BMS scores through 28dpi; lesion size (GFAP- area), fibroblast (PDGFRb+) density, and myeloid (Cd11b+) cell density at 28dpi; and fibroblast (PDGFRb+), myeloid (Cd11b+), and astrocyte (GFAP+) proliferation (EdU+) at 4dpi.
Moderate contusion injury, C57BL/6J mice, mice perfused with 4% PFA, 8mm injury site embedded in OCT, sagittal section taken at 16um, sections stained with antibodies.
Both middle aged and young, naive and 3dpi animals were processed for single cell sequencing. Both middle aged and young, 3dpi animals were processed for spatial sequencing. DATA USAGE NOTES: