Identification of transcription factor Pha-4 binding regions

We identified the DNA binding sites for transcription factors Pha-4 in C. elegans. Transcription factor genes were tagged with GFP and their expression examined throughout the C. elegans life cycle. The binding sites were determined using chromatin immunoprecipitation with anti-GFP antibody followed by illumina high-throughput sequencing (ChIP-seq). For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Two growth stages of pha-4 transgenic worm were studied including embryo and starved L1.