Sequence of antisense oligonucleotides for LuxS and the sequence of related primers. Pyrancoumarin Derivative LP4C Targeting of Pyrimidine de novo Synthesis Pathway Inhibits MRSA Biofilm and Virulence

Supplementary Table 1 Primers of RT-PCR Gene Primer sequence (5’-3’) carA Forward GACAGCGGCGTGGTGATGATC Reverse CTTGTGAGTGGCTCGCAGGTTG carB Forward CGTGCCGTTCGTCTCCAAGTG Reverse GCGGGATGATTTCCTGGGTGAAG pyrB Forward ACCGTGGCGTGGAGATCGAG Reverse GCTGGCGTTGGGTGTTCTGG pyrC Forward AACTCGGACTCTGGCGTGACC Reverse GTGCTCGTCGATGAACTGCTTCTC pyrE Forward GCGGTGATCGACAGCGGAATC Reverse GTGGTCCTTGGCTTCCTTGCG pyrF Forward CGACCTGAAATTCCACGACATCCC Reverse GTTGACCATCCACACGCCCATC luxS Forward AAGTCCTATGGGTTGCCAAACTGG Reverse GCAGGCACTTCAGTAGCATTTAGC saeR Forward CGCCTTAACTTTAGGTGCAGATGAC Reverse ACGCATAGGGACTTCGTGACCATT sigB Forward GGTGCCATAAATAGATTCGATATGTCCTT Reverse CTTTTGATTTCACCGATTACAGTAGGTACT cidA Forward AGCGTAATTTCGGAAGCAACATCCA Reverse CCCTTAGCCGGCAGTATTGTTGGTC icaA Forward GTTGTCGACGTTGGCTACTG Reverse ATGGCAAGCGGTTCATACTT atlA Forward AACAGCACCAACGGATTAC Reverse CATAGTCAGCATAGTTATTCATTG psmα Forward GGCCATTCACATGGAATTCGTAG Reverse TAGCCATCGTTTTGTCCTCCTG psmβ Forward GGACTAGCAGAAGCAATCGCA Reverse CCTAGTAAACCCACACCGTTAGC gyrB Forward GGAGGTAAATTCGGAGGT Reverse CTTGATGATAAATCGTGCCA Supplementary Table 2 Sequence of antisense oligonucleotides for LuxS Oligonucleotides Sequence (5’-3’) Oligo 1 T*A*G*C*G*G*G*A*A*C*G*A*T*G*G*A*A*G*G*A Oligo 2 T*A*T*G*G*A*T*A*T*G*C*C*C*G*G*A*T*T*G*C Oligo 3 G*G*G*T*T*G*C*C*A*A*A*C*T*G*G*T*T*T*C*T