Transcriptional profiling of Bone Marrow Derived Dendritic Cells (BMDC) stimulated with zymosan or bacterial spent medium

We reported transcriptional characterization of BMDCs stimulated by filter-sterilized spent medium of Parabacteroides goldsteinii ASF519 (ASF519 SSM) alone or in comination with zymosan. We identified that ASF519 SSM-induced differentially expressed genes have a wide association with the regulation of immune functions, cell communication, apoptosis. We observed that ASF519 SSM induced transcriptomic changes towards immune tolerance, such as a dramatic induction of tolerogenic Il10, lipocalin-2(Lcn2) and indoleamine 2, 3-dioxygenases (Ido1/2), and a reduction of the co-stimulatory molecule Cd40 and inflammatory cytokines, including Il12b, Il23a, and Il1f9 (or Il-36 gamma). Bulk RNA sequencing of BMDCs post stimulation with either ASF519 SSM or uninoculated bacterial broth alone, or in combination with zymosan.