Cut&RUN P300 BA Primary mouse hepatocytes

Pre-processed CUT&RUN files for P300 were normalized to effective genome size.For each file, background signal was calculated in heterochromatin regions using negativeATAC-seq signal mask, and subsequently removed from the overall coverage.

Peak calling on each replicate was performed using MACS252 v.2.2.7.1, and theconsensus overlapping peaks between all replicates were considered as reproducible for thecorresponding dataset. Number of overlapping peaks between conditions was calculated in Rwith subsetByOverlaps (GenomicRanges v.1.54.1)57, and nearest genes were annotated topeaks using biomaRt. Promoter regions of those genes (± 1kb TSS) were extracted using the Rsubread v.2.16.0) package, and the CUT&RUN signal was quantified with multiBigwigSummary from deepTools package.