Diversified sequences of peptide epitope for same-RNA recognition.
收藏PubMed Central1993-11-01 更新2026-05-16 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC47710/
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资源简介：
We replaced an essential RNA-binding, 30-amino acid helix-loop in an Escherichia coli tRNA synthetase with an inactive and simplified "generic" sequence having 23 of the 30 amino acids as alanine and serine. Wild-type residues were restored in random combinations to generate a library with a sequence complexity of about 1.9 x 10(7). Active molecules were obtained by genetic selection at a frequency of approximately 1% and contained variants with as many as 11 alanine/serine replacements and a total of 17 alanine/serine residues. These variants have activities which are thermodynamically competitive with that of the native protein and therefore are functionally and, most likely, conformationally equivalent. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1993-11-01
