Relative cell death of HUVEC cultures exposed to cilengitide or EMD135981.
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HUVEC were cultured for 18 hours on vitronectin (αVβ3 ligand), fibronectin (α5β1>αVβ3 ligand) and collagen I (α1β1 and α2β1 ligand) before they were exposed for 4 hours to EMD135981 or cilengitide. Cells were collected and Sub-G1 DNA content determined by propidium iodide staining and flow cytometry analysis. Results are expressed as percent increase of cell death relative to untreated conditions. Cilengitide increased HUVEC cell death on vitronectin at high and low protein coating concentrations, while on fibronectin and collagen it only did it at low protein coating concentrations. (n = 3).
创建时间：
2013-02-21
