Drought adaptation modifies soil respiration and microbial community responses to phytohormones

Datasets used in: Sayer EJ, Crawford JA, Edgerley J, Askew AP, Hahn CZ, Whitlock R and Dodd IC (2021) Adaptation to chronic drought modifies soil microbial community responses to phytohormones. Communications Biology MS COMMSBIO-20-2294A

File 1: SayerEtAl2021_CommsBiol_SoilResp.csv

Respiration rates (CO2 efflux) following phytohormone additions at three molar concentrations or a standard root exudate solution, applied to soils from long-term climate change treatments at the Buxton Climate Change Impacts Study, UK. Respiration rates were measured using the Microresp method during 6-h incubations.

Column headings:
Block -> replicate block, determined by original experimental design in the field
Climate.Plot -> climate treatment applied to field plots
RE.add -> addition of standard root exudate solution (RE) or no addition (noRE)
Hormone.Trt -> phytohormone treatment applied to the soils, where ABA is abscisic acid, ACC is 1-aminocyclopropane-1-carboxylic acid and JA is jasmonic acid
Concentration-> molar concentration of the phytohormone solutions applied to soils, where 0M is a procedural control (deionised water)
CO2efflux->total soil respiration in micrograms (ug) C

File 2: SayerEtAl2021_CommsBiol_PLFAs.csv
Soil properties and soil microbial biomarker groups in field soils (inc.Time = 0) and soil microbial biomarker groups after 24h incubation with phytohormone additions at 1M concentration; soils were sampled from long-term climate change treatments at the Buxton Climate Change Impacts Study, UK; biomarker functional groups were determined by phospholipid fatty acid analysis and are given as relative abundances.

Column headings:
Block -> replicate block, determined by original experimental design in the field
Climate.Plot -> climate treatment applied to field plots
Hormone.Trt -> phytohormone treatment applied to the soils at 1M concentration, where ABA is abscisic acid, ACC is 1-aminocyclopropane-1-carboxylic acid and JA is jasmonic acid; CT is field soils before incubation and H2O is a procedural control (incubated with deionised water only).
Inc.Time -> incubation time in hours, where 0 is field soils (pre-incubation)
Total.Biomass -> total PLFA biomass in nM/g soil
AM.Fungi-> arbuscular mycorrhizal fungi (% relative abundance)
Gram.Negative -> Gram negative bacteria (% relative abundance)
Eukaryote-> Eukaryotes (% relative abundance)
Fungi -> saprophytic fungi (% relative abundance)
Gram.Positive -> Gram positive bacteria (% relative abundance)
Actinomycetes -> Actinomycetes (% relative abundance)
FBratio -> Fungal to bacterial ratio
nMfungi -> saprophytic fungal biomass in nM/g soil
nMbacteria -> bacterial biomass in nM/g soil
Gram.Ratio -> ratio of Gram positive to Gram negative bacteria
SWC.percent -> soil water content (%) at the time of sampling
ExtrC.mg.g -> K2SO4-extractable soil carbon (mg/g dry weight)
ExtrN.mg.g-> K2SO4-extractable soil nitrogen (mg/g dry weight)

所使用的数据集包括：Sayer EJ, Crawford JA, Edgerley J, Askew AP, Hahn CZ, Whitlock R 和 Dodd IC (2021) 的研究论文《长期干旱适应性对土壤微生物群落对植物激素响应的影响》。文件1：SayerEtAl2021_CommsBiol_SoilResp.csv，记录了在三种摩尔浓度的植物激素添加或标准根分泌物溶液（应用于英国布克斯顿气候变化影响研究站长期气候变化处理的土壤中）后的呼吸速率（二氧化碳通量）。呼吸速率通过Microresp方法在6小时的培养过程中进行测量。列标题包括：
Block -> 实验设计中的重复小区，由原始田间实验设计确定
Climate.Plot -> 应用于田间小区的气候处理
RE.add -> 标准根分泌物溶液（RE）的添加或无添加（noRE）
Hormone.Trt -> 应用于土壤的植物激素处理，其中ABA为脱落酸，ACC为1-氨基环丙烷-1-羧酸，JA为茉莉酸
Concentration -> 应用于土壤的植物激素溶液的摩尔浓度，其中0M为程序控制（去离子水）
CO2efflux -> 总土壤呼吸量，以微克（ug）碳为单位

文件2：SayerEtAl2021_CommsBiol_PLFAs.csv，记录了田间土壤及其在24小时与1M浓度植物激素添加后的土壤微生物生物标志物组；土壤样品取自英国布克斯顿气候变化影响研究站的长期气候变化处理；生物标志物功能组通过磷脂脂肪酸分析确定，并以相对丰度给出。列标题包括：
Block -> 实验设计中的重复小区，由原始田间实验设计确定
Climate.Plot -> 应用于田间小区的气候处理
Hormone.Trt -> 在1M浓度下应用于土壤的植物激素处理，其中ABA为脱落酸，ACC为1-氨基环丙烷-1-羧酸，JA为茉莉酸；CT为培养前的田间土壤，H2O为程序控制（仅与去离子水一起培养）
Inc.Time -> 培养时间（小时），其中0为田间土壤（预培养）
Total.Biomass -> 总PLFA生物量以nM/克土壤为单位
AM.Fungi->丛枝菌根真菌（%相对丰度）
Gram.Negative -> 革兰氏阴性细菌（%相对丰度）
Eukaryote-> 真核生物（%相对丰度）
Fungi -> 腐生真菌（%相对丰度）
Gram.Positive -> 革兰氏阳性细菌（%相对丰度）
Actinomycetes ->放线菌（%相对丰度）
FBratio -> 真菌与细菌比率
nMfungi -> 腐生真菌生物量以nM/克土壤为单位
nMbacteria -> 细菌生物量以nM/克土壤为单位
Gram.Ratio -> 革兰氏阳性与革兰氏阴性细菌比率
SWC.percent -> 采样时的土壤含水量（%）
ExtrC.mg.g -> K2SO4可提取土壤碳（mg/干重克）
ExtrN.mg.g-> K2SO4可提取土壤氮（mg/干重克）