MOESM1 of F4+ ETEC infection and oral immunization with F4 fimbriae elicits an IL-17-dominated immune response

Additional file 1. F4 + ETEC number detected in the faeces after F4 + ETEC infection. Piglets were orally inoculated with either 1010 CFU F4+ ETEC (Pig 4 to 6) in 10Â mL PBS or only PBS (Pig 1 to 3) as control at day 0 and 1. Faecal samples were collected from day 0 to 4 and inoculated onto blood agar plates and then the number of F4-specific hemolytic E. coli colonies was determined by dot blotting the colonies on nitrocellulose membranes and detecting F4 fimbriae with an F4ac-specific MAb.