Validation of the anti-infective potential of a polyherbal ''Panchvalkal'' preparation, and elucidation of the molecular basis underlining its efficacy

We undertook to validate the therapeutic use of a polyherbal preparation described in Ayurved as ‘Panchvalkal’, which has been suggested for treatment of microbial infections. We tested this formulation available in market as Pentaphyte P-5®, against Pseudomonas aeruginosa, for its quorum sensing (QS) modulatory potential. Following demonstration of its in vitro QS modulatory potential, we assayed it for in vivo efficacy using the nematode Caenorhabditis elegans as a model host. This polyherbal formulation conferred notable survival benefit when the nematode worm was challenged with the test pathogen. Todecipher the molecular basis of its efficacy, whole transcriptome analysis of P. aeruginosa exposed to ‘Panchvalkal’ was done, its gene expression profile inpresence of ‘Panchvalkal’ was compared with that in its absence. This polyherbal formulation also enhanced the susceptibility of P. aeruginosa to antibiotics like gentamicin, tetracycline, and cephalexin. This study is a good demonstration of the role of bacterial QS machinery as an important target for development of new antimicrobials/ anti-infectives, and also of the effective use of moderngenomics for validation of ayurvedic prescriptions i.e. ayuromics.