Photosynthetic parameters of the D1-R238E tobacco mutant.

Thylakoids of mutated tobacco lines in which arginine 238 of the D1 protein was modified to glutamate (R238E) display similar photosynthetic characteristics to thylakoids of WT and WT-aadA tobacco lines.*O2 evolution was determined with a Clark type electrode, with DCBQ as the exogenous electron acceptor.§The maximum light-saturated linear electron transport capability (ETR), chlorophyll (Chl) content and a/b ratio and the content of major photosynthetic complexes photosystem I (PSI), photosystem II (PSII), and the cytochrome b6f were determined spectroscopically, as detailed in the Experimental section. Values are average of six independent experiments, and presented ± standard error.Photosynthetic parameters of the D1-R238E tobacco mutant.