TR4 and BCL11A Repress gamma-globin Transcription via Independent Mechanisms

Nuclear receptor TR4 was previously shown to bind to the -117 position of the gamma-globingene promoters in vitro, which overlaps with the BCL11A binding site recently described. Therole of TR4 in human gamma-globin gene repression has not been extensively characterized in vivo,and the relationship between TR4 and BCL11A binding to the gamma-globin promoters remainedelusive. We showed in vitro that TR4 and BCL11A competitively bind to overlapping but distinctconsensus sequences which both include the -117 position of the gamma-globin promoter. We showhere that TR4 represses gamma-globin transcription and HbF accumulation in a BCL11A-independentmanner. We characterized the chromatin occupancy of TR4 within the beta-globin locus incomparison to BCL11A and found that both bind to the hypersensitive sites avidly but onlyBCL11A binds to the gamma-globin promoters at significant levels, suggesting that BCL11A is thepredominant repressor acting through the -117 gamma-globin promoter sequence in vivo. These dataresolve an important discrepancy in the literature, and thus helps to clarify possible approachesto treatments for sickle cell disease or beta-thalassaemia.