Double nucleotide polymorphisms within the katG gene.

Double nucleotide polymorphisms within the katG gene in colonies isolated from slow growth (Culture INH69.3) and fast growth cultures (Culture INH23.3) after 12–13 MGT were identified by Sanger sequencing. Five colonies at each growth rate were found to contain a double mutation in katG; some of these double mutations included a mutation at katG codon Ser315, which are indicated in brackets. The notation used for each mutation is as follows: original nucleotide followed by the nucleotide number and finally the new nucleotide. Some of the katG codon Ser315 mutations will have shifted by a nucleotide because of a deletion (“del”) or insertion (“ins”) upstream of the katG codon Ser315. Corresponding amino acid changes or effects at each position are also indicated. In cases where an amino acid alteration is indicated by an asterisk there was a truncation of the gene.Double nucleotide polymorphisms within the katG gene.